Goat anti-Rat IgG2a Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN7233172

Quick Overview for Goat anti-Rat IgG2a Antibody (Alkaline Phosphatase (AP)) (ABIN7233172)

Target: IgG2a

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Isotype: IgG

Product Details

Reactivity: Rat

Host: Goat

Purpose: Anti-Rat IgG2a-Alk Phos Goat Polyclonal Antibody

Specificity: Antibody is affinity-purified on rat IgG2a and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgG2a of other species.

Cross-Reactivity: Rat

Immunogen: Rat IgG2a

Application Details

Application Notes: Immunoblotting: use at a dilution of 1:1,000-1:10,000.

Immunohistochemistry: use at a dilution of 1:100-1:1,000

Immunocytochemistry: use at a dilution of 1:100-1:1,000

ELISA: use at a dilution of 1:1,000-1:10,000.

These are recommended dilutions.

End users should determine optimal dilutions for their applications. Prepare working dilutions immediately before use.

Comment:

Dilute in PBS or in medium that is identical to that used in the assay system.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 0.5 mg/mL

Buffer: 50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2 % BSA and 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: This antibody is stable for at least 1 year at 2-8°C.

Target Details

Target: IgG2a

Target Type: Antibody